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Title   폐의 비소세포암에서 p53유전자 돌연변이와 TGF-B에 관한 분자생물학적 연구 ( TGF-beta-1 Expression and p53 Mutation in Non-small Cell Carcinomas of the Lung )
Publicationinfo   1997 Jan; 029(06): 1022-1032.
Key_word   TGF-beta-l, p53, Non-small cell carcinoma
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Abstract   Purpose: TGF-beta-1 is actually a major growth inhibitor for most cell types. We assumed that the loss of TGF-beta-1 would be occurred during carcinogenesis of the lung. Also, the mutation and expression of p53 have been known to be major moleclar change of non-small cell carcinoma of the lung. So, the relationship between the mutation of p53 and the expression of TGF-beta-1 in the non-small cell carcinomas were evaluated. Materials and Methods: In 43 non-small cell carcinoma and normal tissue of the lung, their TGF-beta-1 mRNA were measured by RT-PCR and p53 was studied by SSCP and Western blotting assay. Results: p53 mutation rate in non-small cell carcinomas of the lung (48.4%) was much more frequent than the normal control group (14.3%). The expression rate of TGF-beta-1 in lung carcinomas, especially squamous'cell carcinoma (71.4%), was much higher than the norrnal control group (42.9%). p53 mutation and TGF-beta-1 mRNA in the lung carcinomas were not strongly correlated. Conclusion: It suggests that high expression rate of TGF-beta-1 and p53 mutation are associated with carcinogenesis of non-small cell carcinoma of the lung. High expression rate of TGF-beta-1 in the lung carcinomas can be partly explained by the fact that TGF-beta-1 have capacity to control the production of rnany components of the extracellular matrix and enhance angiogenesis in favor of tumor growth despite of their inhibitory effects of cell gro;vth. However, additional research is required to determine the exact role of TGF-beta-1 in carcinogenesis of the lung.
저 자   김한겸(Han Kyum Kim),김영식(Young Sik Kim),박설희(Sul Hee Park),나영순(Young Soon Na),강용구(Yong Koo Kang),한정호(Jung Ho Han),박미자(Mi Ja Park)김인선(In Sun Kim),